Three-dimensional arbitrary trajectory scanning photoacoustic microscopy.
نویسندگان
چکیده
We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2 ) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method.
منابع مشابه
All-optical photoacoustic microscopy
Three-dimensional photoacoustic microscopy (PAM) has gained considerable attention within the biomedical imaging community during the past decade. Detecting laser-induced photoacoustic waves by optical sensing techniques facilitates the idea of all-optical PAM (AOPAM), which is of particular interest as it provides unique advantages for achieving high spatial resolution using miniaturized embod...
متن کاملHybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging.
Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical-mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facil...
متن کاملPhotoimprint photoacoustic microscopy for three-dimensional label-free subdiffraction imaging.
Subdiffraction optical microscopy allows the imaging of cellular and subcellular structures with a resolution finer than the diffraction limit. Here, combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for subdiffraction photoacoustic imaging of both fluorescent and nonfluorescent samples. Our method is based on a dou...
متن کاملFully motorized optical-resolution photoacoustic microscopy.
We have developed fully motorized optical-resolution photoacoustic microscopy (OR-PAM), which integrates five complementary scanning modes and simultaneously provides a high imaging speed and a wide field of view (FOV) with 2.6 μm lateral resolution. With one-dimensional (1D) motion-mode mechanical scanning, we measured the blood flow through a cross section of a blood vessel in vivo. With two-...
متن کاملMicrovascular quantification based on contour-scanning photoacoustic microscopy.
Accurate quantification of microvasculature remains of interest in fundamental pathophysiological studies and clinical trials. Current photoacoustic microscopy can noninvasively quantify properties of the microvasculature, including vessel density and diameter, with a high spatial resolution. However, the depth range of focus (i.e., focal zone) of optical-resolution photoacoustic microscopy (OR...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of biophotonics
دوره 8 4 شماره
صفحات -
تاریخ انتشار 2015